ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of propofol, midazolam and thiopental sodium on outcomes and amino acid accumulation in focal cerebral ischemia-reperfusion in rats.</p><p><b>METHODS</b>Male Sprague Dawley (SD) rats were scheduled to undergo 3-hour middle cerebral artery occlusion by intraluminal suture and 24-hour reperfusion. Neurologic outcomes were scored on a 0-5 grading scale. Infarct volume was shown with triphenyltetrazolium chloride staining and measured by an image analysis system. Concentrations of various amino acids (aspartate, glutamate, glycine, taurine, and gama-aminobutyric acid) were measured after 3 hours of reperfusion using high performance liquid chromatography. Propofol, midazolam and thiopental sodium were given intraperitoneally at the beginning of reperfusion.</p><p><b>RESULTS</b>Both propofol and midazolam attenuated neurological deficits and reduced infarct and edema volumes. Propofol showed better neurological protection than midazolam while thiopental sodium did not exhibit any protective effect. Both propofol and midazolam decreased excitatory amino acids accumulation, while propofol increased gama-aminobutyric acid accumulation in ischemic areas in reperfusion.</p><p><b>CONCLUSION</b>Propofol and midazolam, but not thiopental sodium, may provide protective effects against reperfusion induced injury in rats subjected to focal cerebral ischemia. This neurological protection may be due to the acceleration of excitatory amino acids elimination in reperfusion.</p>
Subject(s)
Animals , Male , Rats , Adenosine Triphosphate , Metabolism , Brain , Metabolism , Brain Edema , Drug Therapy , Brain Ischemia , Metabolism , Excitatory Amino Acids , Metabolism , Midazolam , Pharmacology , Myocardial Infarction , Drug Therapy , Neuroprotective Agents , Pharmacology , Propofol , Pharmacology , Rats, Sprague-Dawley , Reperfusion Injury , Thiopental , PharmacologyABSTRACT
AIM: To investigate the effects of propofol, midazolam and thiopental sodium on neurologic and histologic outcome from focal cerebral ischemia-reperfusion in a rat model of reversible middle cerebral artery occlusion (MCAO). METHODS: Male SD rats were scheduled to undergo 3 h MCAO by intraluminal suture and 24 h reperfusion. Neurologic outcome was scored with a 0-5 grading scale. Infarct volume was shown with TTC staining and measured by image analysis system. Ultrastructure of the tissues taken from the brim of the damaged area was examined under electron microscope. RESULTS: Both propofol and midazolam could attenuate neurologic deficits, reduce infarct and edema volumes, and ameliorate ultrastructure damage at the brim of lesion. Propofol showed better neuroprotection than midazolam while thiopental sodium did not exhibit protective effect. CONCLUSION: Propofol and midazolam, but not thiopental sodium, can provide protective effects against reperfusion induced injury in rats subjected to focal cerebral ischemia.
ABSTRACT
The experiment was designed to investigate the effect of salvia miltiorrhiza (Danshen ) and its effective composition, phenyl lactic acid (Danshensu )on the hypoxic pulmonary vasoconstriction (HPV). Acute hypoxia was induced by replacing mixed gas 95%O2+ 5 %CO2 with 95%N2 + 5 %CO2. Hypoxic pulmonary vasoconstriction was diminished by injectic Danshen, Injectic Danshen compound and Danshensu with concentration dependently. Tile effect of Danshensu was the most,and Injectic Danshen Co was second by comparison. The results suggested that Danshen can antagonist HPV which was mainly produced by Danshensu, one of the effective composition of Danshen,and that more potent inhibitory action on HPV was by Injectio Danshen compound compared with Injectio Danshen,and this may be related to the addition of Dalbergia odorifera , the other composition of Injectic Danshen compound.